- 品名： PE/Dazzle? 594 anti-mouse CD133 Antibody
- 型號： 141211
- 產(chǎn)品詳情
- 規(guī)格參數(shù)
Description
CD133 (also known as prominin-1 and AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.
Clone
Regulatory Status
RUO
Other Names
Prominin-1, AC133
Isotype
Rat IgG2a, λ
Ave. Rating
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 141211 | 25 μg | $159 | ||||
| 141212 | 100 μg | $370 | |||
Description
CD133 (also known as prominin-1 and AC133) is a 120 kD pentaspan glycoprotein with 5 transmembrane domains which localize to cellular protrusions. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to hematopoietic stem cells, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. It also has been detected at low levels in the kidney, pancreas, placenta, and fetal liver tissue. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.
Product Details
Isotype Control
Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD133-expressing-GFP-RBL2H3 plus CFA as adjuvant.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle? 594 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 μl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle? 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)Application References
(PubMed link indicates BioLegend citation)Zhang Y, et al. 2012. Diabetes. 61:2114. PubMed
RRID
AB_2566009 (BioLegend Cat. No. 141211)
AB_2566010 (BioLegend Cat. No. 141212)
