- 品名: 普諾賽MT-4 (人T細胞白血病細胞)
- 型號: CL-0655
- 產(chǎn)品詳情
- 規(guī)格參數(shù)
產(chǎn)品信息
名稱 | MT-4 (人T細胞白血病細胞) (STR鑒定正確)(僅提供凍存管) |
背景描述 | Derived from a 50 year old Japanese male with generalised lymphadenopathy and heptosplenomegaly (Adult T-cell Leukaemia) by co-culture of his peripheral leukocytes with male umbilical cord lymphocytes. The cells carry HTLV-1 and support the growth of HIV. The cells should be handled under laboratory containment level 3 conditions. |
種屬 | 人 |
組織來源 | 淋巴細胞 |
生長特性 | 懸浮細胞 |
細胞形態(tài) | 淋巴細胞樣 |
細胞類型 | 腫瘤細胞 |
腫瘤類型 | 淋巴瘤細胞 |
生長培養(yǎng)基 | RPMI-1640+10% FBS+1% P/S |
推薦傳代比例 | 3×10^5-5×10^5cells/mL |
推薦換液頻率 | 2~3次/周 |
凍存條件 | 凍存液:55% 基礎培養(yǎng)基+40%FBS+5%DMSO溫度:液氮 |
培養(yǎng)條件 | 氣相:空氣,95%;CO2,5% |
注意事項 | 該細胞為懸浮細胞,請注意離心收集細胞懸液;請勿直接倒掉細胞培養(yǎng)液。 |
STR鑒定
STR位點信息
Amelogenin X,Y CSF1PO 11,12 D2S1338 18,23 D3S1358 17 D5S818 10,11 D7S820 8,10 D8S1179 10,15 D13S317 12 D16S539 9,12 D18S51 13 D19S433 14.2,15.2 D21S11 28,30.3 FGA 23 PentaD 10,13 PentaE 5,15 TH01 7 TPOX 11 vWA 17,18 D6S1043 12 D12S391 18,21 D2S441 10
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