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產(chǎn)品目錄

普諾賽MIA PaCa-2 (人胰腺癌細(xì)胞)

品名: 普諾賽MIA PaCa-2 (人胰腺癌細(xì)胞)
型號(hào): CL-0627
  • 產(chǎn)品詳情
  • 規(guī)格參數(shù)

產(chǎn)品信息

名稱MIA PaCa-2 (人胰腺癌細(xì)胞) (STR鑒定正確)
別稱MIA-PaCa-2; MIA-PACA-2; MIA-Pa-Ca-2; MIA Paca2; MIA PaCa2; MiaPaCa-2; MIAPACA-2; MiaPaca.2; MiaPaCa2; Miapaca2; MIAPaCa2; MIAPACA2; Mia PACA 2; MIAPaCa-2; PaCa2
背景描述The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male.
種屬
年齡(性別)男;65歲
生長特性半貼半懸
細(xì)胞形態(tài)上皮樣貼壁細(xì)胞,伴有圓形漂浮細(xì)胞
細(xì)胞類型腫瘤細(xì)胞
腫瘤類型胰腺癌細(xì)胞
生物安全等級(jí)1
生長培養(yǎng)基DMEM10%FBS2.5%HS1%P/S
推薦傳代比例1:3-1:8
推薦換液頻率2~3次/周
倍增時(shí)間26 hours (PubMed=25984343); 25.7 +- 4.3 hours (PubMed=27067801); 40 hours, 18 hours, in serum-free medium (PubMed=23386380); ~40 hours (ATCC); ~30-40 hours (DSMZ)
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
基因表達(dá)情況human colony stimulating factor, subclass I (CSF-I); plasminogen activator
保藏機(jī)構(gòu)ATCC; CRL-1420DSMZ; ACC-733ECACC; 85062806JCRB; JCRB0070
STR鑒定
  • STR位點(diǎn)信息

    AmelogeninX
    CSF1PO10
    D2S133825
    D3S135816
    D5S81812,13
    D7S82012
    D8S117916
    D13S31712,13
    D16S53910,13
    D18S5112
    D19S43315
    D21S1129,31.2
    FGA22
    PentaD12,16
    PentaE13,18
    TH019,10
    TPOX9
    vWA15
    D6S104312
    D12S39119
    D2S44114


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