- 品名: 普諾賽HTR-8/Svneo (人絨毛膜滋養(yǎng)層細(xì)胞)
- 型號(hào): CL-0599
- 產(chǎn)品詳情
- 規(guī)格參數(shù)
產(chǎn)品信息
名稱 | HTR-8/Svneo (人絨毛膜滋養(yǎng)層細(xì)胞) (暫不出售) |
別稱 | HTR-8/SV neo; HTR-8/SV-neo; HTR8/SVneo; HTR8svn |
背景描述 | The HTR-8/SVneo cell line was derived by transfecting the cells that grew out of chorinic villi explants of human first-trimester placenta with the gene encoding for simian virus 40 large T antigen. |
種屬 | 人 |
年齡(性別) | 女;6-12周胚胎 |
組織來源 | 胎盤 |
生長特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
細(xì)胞類型 | 轉(zhuǎn)化細(xì)胞系 |
生物安全等級(jí) | 2 |
生長培養(yǎng)基 | RPMI-1640+5% FBS+1% P/S |
推薦傳代比例 | 1:3-1:5 |
推薦換液頻率 | 2~3次/周 |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮 |
培養(yǎng)條件 | 氣相:空氣,95%;CO2,5%溫度:37℃ |
致瘤性 | No (tested in soft agar and nude mice). |
抗原表達(dá)情況 | cytokeratin-7+; insulin-like growth factor (IGF)-II+; NDOG-5 (placental alkaline phosphatase (PLAP) antibody) +; proliferating cell nuclear antigen (PCNA)+; human leukocyte antigen framework antigen (W6/32)+; integrin subunits alpha 1, alpha 3, alpha |
保藏機(jī)構(gòu) | ATCC; CRL-3271 |
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