- 品名: 普諾賽SNB-19 (人膠質(zhì)瘤細(xì)胞)
- 型號: CL-0582
- 產(chǎn)品詳情
- 規(guī)格參數(shù)
產(chǎn)品信息
名稱 | SNB-19 (人膠質(zhì)瘤細(xì)胞) (U-251 MG污染細(xì)胞系,暫不供應(yīng)) |
別稱 | SNB.19; SNB19 |
背景描述 | Established from the surgical resection of a left parieto-occipital glioblastoma from a 47-year-old man in 1980; cells were described in the literature to secrete plasminogen activator, to be clonogenic in soft agar and to be tumorigenic in nude mice; DNA fingerprinting showed unequivocally that SNB-19 is a subclone of the astrocytoma cell line U-251 MG)。(STR檢測位點(diǎn)同U-251MG) |
種屬 | 人 |
年齡(性別) | 男;75歲 |
組織來源 | 星形膠質(zhì)瘤(U-251MG) |
生長特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 成纖維細(xì)胞樣 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 膠質(zhì)瘤細(xì)胞 |
生物安全等級 | 1 |
生長培養(yǎng)基 | DMEM+10% FBS+1% P/S |
推薦傳代比例 | 1:4-1:6 |
推薦換液頻率 | 2~3次/周 |
倍增時(shí)間 | ~24-36 hours |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮 |
培養(yǎng)條件 | 氣相:空氣,95%;CO2,5%溫度:37℃ |
保藏機(jī)構(gòu) | DSMZ; ACC-325 |
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