- 品名: 普諾賽BT-B (人膀胱癌細(xì)胞)
- 型號(hào): CL-0580
- 產(chǎn)品詳情
- 規(guī)格參數(shù)
產(chǎn)品信息
名稱 | BT-B (人膀胱癌細(xì)胞) (Hela污染細(xì)胞系,暫不供應(yīng)) |
背景描述 | These cells were thought to be "stablished from the malignant urinary bladder carcinoma of a 66-year-old Caucasian man (stage pTa G3) in 1989"; same donor as for cell line BT-A; however, DNA fingerprinting and cytogenetic analysis at the DSMZ Established unequivocally that this cell line must be considered de facto a subclone of HELA。(STR檢測(cè)位點(diǎn)同HELA) |
種屬 | 人 |
年齡(性別) | 女;30歲6個(gè)月 |
組織來(lái)源 | 膀胱癌組織 |
生長(zhǎng)特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 膀胱癌細(xì)胞 |
生物安全等級(jí) | 1 |
生長(zhǎng)培養(yǎng)基 | RPMI-1640+10% FBS+1% P/S |
推薦傳代比例 | 1:4-1:6 |
推薦換液頻率 | 2~3次/周 |
倍增時(shí)間 | ~48 hours |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮 |
培養(yǎng)條件 | 氣相:空氣,95%;CO2,5%溫度:37℃ |
保藏機(jī)構(gòu) | DSMZ; ACC-227 |
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