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產(chǎn)品目錄

普諾賽BT-B (人膀胱癌細(xì)胞)

品名: 普諾賽BT-B (人膀胱癌細(xì)胞)
型號(hào): CL-0580
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產(chǎn)品信息

名稱BT-B (人膀胱癌細(xì)胞) (Hela污染細(xì)胞系,暫不供應(yīng))
背景描述These cells were thought to be "stablished from the malignant urinary bladder carcinoma of a 66-year-old Caucasian man (stage pTa G3) in 1989"; same donor as for cell line BT-A; however, DNA fingerprinting and cytogenetic analysis at the DSMZ Established unequivocally that this cell line must be considered de facto a subclone of HELA。(STR檢測(cè)位點(diǎn)同HELA)
種屬
年齡(性別)女;30歲6個(gè)月
組織來(lái)源膀胱癌組織
生長(zhǎng)特性貼壁細(xì)胞
細(xì)胞形態(tài)上皮細(xì)胞樣
細(xì)胞類型腫瘤細(xì)胞
腫瘤類型膀胱癌細(xì)胞
生物安全等級(jí)1
生長(zhǎng)培養(yǎng)基RPMI-164010% FBS1% P/S
推薦傳代比例1:4-1:6
推薦換液頻率2~3次/周
倍增時(shí)間~48 hours
凍存條件凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮
培養(yǎng)條件氣相:空氣,95%;CO2,5%溫度:37℃
保藏機(jī)構(gòu)DSMZ; ACC-227


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