- 品名: 普諾賽KYSE-30 (人食管鱗癌細(xì)胞)
- 型號: CL-0577
- 產(chǎn)品詳情
- 規(guī)格參數(shù)
產(chǎn)品信息
名稱 | KYSE-30 (人食管鱗癌細(xì)胞) (STR鑒定正確) |
別稱 | KYSE 30; KYSE30; KYSE0030 |
背景描述 | Derived from well differentiated invasive esophageal squamous cell carcinoma resected from middle intra-thoracic esophagus of a 64-year-old Japanese man prior to treatment; cell line Established from tumor cells heterotransplanted into athymic mice; described in the literature to be heterotransplantable in athymic mice and to carry p53 mutation and amplification of cERB B, MYC and CYCLIN D1 |
種屬 | 人 |
年齡(性別) | 女;64歲 |
組織來源 | 食管鱗狀上皮 |
生長特性 | 貼壁細(xì)胞 |
細(xì)胞形態(tài) | 上皮細(xì)胞樣,帶有長的偽足 |
細(xì)胞類型 | 腫瘤細(xì)胞 |
腫瘤類型 | 食管癌細(xì)胞 |
生物安全等級 | 1 |
生長培養(yǎng)基 | RPMI-1640+Ham's F-12+2mM L-Glutamine+10% FBS+1% P/S |
推薦傳代比例 | 1:3-1:5 |
推薦換液頻率 | 2~3次/周 |
倍增時(shí)間 | ~20-30 hours |
凍存條件 | 凍存液:55% 基礎(chǔ)培養(yǎng)基+40%FBS+5%DMSO溫度:液氮 |
培養(yǎng)條件 | 氣相:空氣,95%;CO2,5%溫度:37℃ |
保藏機(jī)構(gòu) | DSMZ; ACC-351 ECACC; 94072011 JCRB; JCRB0188 |
STR鑒定
STR位點(diǎn)信息
Amelogenin X CSF1PO 10 D2S1338 23 D3S1358 15,16 D5S818 11 D7S820 11,11.3 D8S1179 12,15 D13S317 9 D16S539 10,12 D18S51 14 D19S433 14.2,15.2 D21S11 28 FGA 24 PentaD 12 PentaE 13 TH01 9 TPOX 9 vWA 16,18,19 D6S1043 11,20 D12S391 17,19 D2S441 11
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